ABSTRACT
For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).
Subject(s)
DNA, Plant/isolation & purification , Genetic Variation , Polymorphism, Restriction Fragment Length , Random Amplified Polymorphic DNA Technique , Terminalia/genetics , DNA, Plant/genetics , Cetrimonium Compounds , Genetic Markers , Genetics, Population , Plants, Medicinal/genetics , Reproducibility of ResultsABSTRACT
In this article, we present some simple yet effective statistical techniques for analysing and comparing large DNA sequences. These techniques are based on frequency distributions of DNA words in a large sequence, and have been packaged into a software called SWORDS. Using sequences available in public domain databases housed in the Internet, we demonstrate how SWORDS can be conveniently used by molecular biologists and geneticists to unmask biologically important features hidden in large sequences and assess their statistical significance.